Table 2. Kinetics of Enzymes Encoded by the RspABD Operon.
protein | substrate | kcat (s-1) | kcat/KM (M–1 s–1) |
---|---|---|---|
RspA7 | d-mannonate | 0.02 ± 0.001 | 1.0 × 10 |
RspB | l-gulonate | 3.9 ± 0.5 | 1.4 × 103 |
RspD | 5-keto-d-mannonate | 30 ± 1.8 | 2.8 × 105 |
protein | substrate | kcat (s-1) | kcat/KM (M–1 s–1) |
---|---|---|---|
RspA7 | d-mannonate | 0.02 ± 0.001 | 1.0 × 10 |
RspB | l-gulonate | 3.9 ± 0.5 | 1.4 × 103 |
RspD | 5-keto-d-mannonate | 30 ± 1.8 | 2.8 × 105 |