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. 2014 Aug 19;53(35):5661–5673. doi: 10.1021/bi500693a

Table 1. Steady-State Kinetic Parameters for Wild-Type RcPutA and BjPutA and Their Variants.

	PRODH^a			P5CDH^b
	k_cat (s^–1)	K_m (mM)	k_cat/K_m (M^–1 s^–1)	k_cat (s^–1)	K_m (μM)	k_cat/K_m (M^–1 s^–1)
RcPutA (wild-type)	20.7 ± 0.4	54.3 ± 2.8	383 ± 4	7.3 ± 0.6	1530 ± 160	4800 ± 1000
RcPutAG1011P	22.4 ± 1.4	10.1 ± 3.2	2200 ± 840	–	–	–
RcPutAE1012A	19.8 ± 0.7	15.3 ± 2.6	1300 ± 270	–	–	–
RcPutAN1014A	4.8 ± 0.2	44.5 ± 6.7	110 ± 21	–	–	–
RcPutAΔ1008–1012	8.9 ± 0.2	17.8 ± 1.8	490 ± 62	–	–	–
RcPutA1–1116	26.9 ± 0.9	36.2 ± 4.5	740 ± 120	4.7 ± 0.2	1560 ± 86	3010 ± 290
BjPutA (wild-type)^c	5.6 ± 0.7	150 ± 11	37 ± 7.4	2.2 ± 0.7	200 ± 6	11000 ± 3830
BjPutAG638P	6.1 ± 1.6	147 ± 82	42 ± 34	–	–	–
BjPutAE639A	1.95 ± 0.24	85 ± 29	23 ± 11	–	–	–
BjPutAN641A	2.5 ± 0.4	100 ± 37	29 ± 15	–	–	–
BjPutAΔ635–639	0.5 ± 0.1	43 ± 14	11 ± 5	–	–	–
BjPutA1–986	10.7 ± 1.2	151 ± 38	71 ± 26	4.4 ± 0.1	275 ± 22	16000 ± 1800

^a

DCPIP assay with proline as the variable substrate and a fixed DCPIP concentration of 75 μM.

^b

P5C is the variable substrate with a fixed NAD⁺ concentration of 200 μM.

^c

Values were determined previously.^4,5