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. 2014 Sep 9;9(9):e106458. doi: 10.1371/journal.pone.0106458

Figure 4. Effect of wogonin on the EKR and AKT signaling in B16-F10 cells.

Figure 4

(A-B) B16-F10 cells were pretreated with different concentrations of wogonin for 24 h, and the cellular extracts were then blotted using specific antibodies. Phosphorylated protein expression of p-ERK and p-AKT was tested. Corresponding to the phosphorylation level, respective total amount of ERK1/2 and AKT were detected. (C) B16-F10 cells were pretreated with wogonin (60 µM) and U0126 (20 µM) for 24 h. The expression of ERK, p-ERK, MMP-2 and Rac1 protein in cells was analyzed by western blotting using specific antibodies. (D) B16-F10 cells were pretreated with wogonin (60 µM) and LY294002 (20 µM) for 24 h. The expression of AKT, p-AKT, MMP-2 and Rac1 protein in cells was analyzed by western blotting using specific antibodies. An anti-β-actin antibody was used to check the proper protein loading. Western blotting was done at least three times. *p<0.05 compared with control; **p<0.01 compared with control.