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. 2014 Sep 9;9(9):e106458. doi: 10.1371/journal.pone.0106458

Figure 6. Wogonin inhibits IGF-1-induced invasion through AKT/PI3K and NF-κB pathways.

Figure 6

B16-F10 cells were pretreated with different concentrations of wogonin with IGF-1 (20 ng/ml) for 24 h. (A) B16-F10 cells were scraped with a pipette tip. After 24 h-treatment, migration was assessed by microscope. (B) Pretreated cells were seeded in the upside of transwell, and the membrane was stained with hematoxylin and eosin after 24 h-incubation. (C) Western blotting analyses of the expression of MMP-2 and AKT/PI3K (PI3K, Rac1, p-AKT and AKT) pathway-related protein was performed. (D) NF-κB pathway-related protein (p-IKKα, IKKα, p-IκBα and IκBα) was determined by western blot assay. All protein was determined with specific antibodies. β-actin antibody was used to check the proper protein loading. Each experiment was done at least three times. *p<0.05 compared with IGF-1-treated group; **p<0.01 compared IGF-1-treated group.