Figure 7. Wogonin inhibits TNF-α-induced invasion through NF-κB pathways.

B16-F10 cells were pretreated with TNF-α (20 ng/ml) and different concentrations of wogonin for 24 h. (A) B16-F10 cells were scraped with a pipette tip before the treatment of wogonin and TNF-α. The strach was photographed by microscope. (B) Pretreated cells were counted and cultured in the upside of transwell coated with the matrigel, and the cells through the matrigel were stained with hematoxylin and eosin after 24 h-incubation. (C) Western blotting analyses of MMP-2 and NF-κB pathway-related protein (p-IKKα, IKKα, p-IκBα and IκBα) was performed. All protein was determined with specific antibodies. β-actin antibody was used to check the proper protein loading. Each experiment was done at least three times. *p<0.05 compared with TNF-α-treated group; **p<0.01 compared with TNF-α-treated group.