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. Author manuscript; available in PMC: 2014 Sep 9.
Published in final edited form as: Methods Mol Biol. 2013;960:411–432. doi: 10.1007/978-1-62703-218-6_31

Fig.2. Pulse/chase analysis of DQ1 molecules in DM-expressing and non-expressing B-LCL.

Fig.2

2.2.93 and 2.2.93-DM cells were pulsed for 1 hr with [35S]-Cys/Met and chased for the indicated time periods. Aliquots of cell lysates, normalized for counts, were immunoprecipitated with SPVL-3 for DQ1 and then analysed by SDS-PAGE [44]. This figure illustrates the preferential recognition of a mature confirmation of DQ1 by SPVL3, which is likely peptide-dependent and is significantly increased in the presence of DM.