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. 2014 Sep 9;9(9):e106547. doi: 10.1371/journal.pone.0106547

Figure 5. Effect of mutations in the C-terminal region of the SBD on binding with PHD3.

Figure 5

HEK293T cells were transfected with the expression plasmids for the indicated proteins. The cells were lysed followed by FLAG immunoprecipitation (IP) of cell lysates. Immunoprecipitates and lysates were then analyzed by western blotting using the indicated antibodies. The [S2]NT[S1-Q121L]CT chimera regained binding equivalent to Siah2 SBD wild type. In contrast, [S2]NT[S1-T160A]CT showed only a small increase in PHD3 binding. FLAG-SBD Siah in the IP was masked by the IgG light chain.