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. 2014 Sep 9;9(9):e106861. doi: 10.1371/journal.pone.0106861

Figure 4. Effect of low dose chronic chlorine exposure on the molecules associated with inflammasome activation.

Figure 4

A, IL-1β levels in the BAL from OVA-sensitized and challenged mice with or without chlorine exposure were measured by ELISA. *p<0.05 vs. OVA only group. B, The mRNA expressions of IL-1β were measured by RT-qPCR after 48 and 96 hrs stimulation with 0.001% NaOCl. *p<0.05 vs. control. C, IL-1β levels were measured using ELISA after low dose NaOCl treatments in AMJ2-C11 mouse macrophage cells. *p<0.05 vs. control. D, Western blot evaluation on the molecules associated with inflammasome activation in the macrophages with and without exposure of NaOCl (IL-1β and caspase-1 in the cell supernatant, and IL-18 in the cell lysates). Values in panels in A, B and C are mean ± SEM. Panel D is a representative of a minimum of three similar experiments.