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. 2014 Sep 9;9(9):e107346. doi: 10.1371/journal.pone.0107346

Figure 4. Homo- and heterointeraction of Aux/IAA proteins in BiFC experiments visualized by confocal microscopy.

Figure 4

(A) Homotypic protein interactions of Aux/IAA proteins were identified for ZmIAA11, ZmIAA15 and ZmIAA33 but not for ZmIAA20 and ZmIAA2. (B) Heterotypic interactions of RUM1 were detected for all five tested Aux/IAA proteins. RUL1 specifically interacted with ZmIAA15 and ZmIAA33. (C) Summary of the quantitative BiFC data obtained by flow cytometry (see Table 2) and the verification of these results by qualitative BiFC experiments surveying protoplasts under a confocal microscope (Figure 4A and B). The arrowhead types are indicating the interacting partners. A full arrowhead describes an interaction of RUM1/RUL1-YFPN with Aux/IAA-YFPC, while a double arrowhead denotes an interaction of RUM1/RUL1-YFPC with Aux/IAA-YFPN. Homotypic interactions of ZmIAA11, ZmIAA15 and ZmIAA33 are highlighted with a double circle.