Table 5. ASCV screening of field samples, sample set B.
Population I 2012 | ASCV | PRV | PMCV | ||||||
HE | HK | PC | HE | HK | PC | HE | HK | PC | |
Fish no. 1 | 30,32 | 27,08 | - | 28,05 | 30,24 | 31,86 | - | - | - |
2 | 25,32 | 20,49 | 31,71 | 29,58 | 29,59 | 31,06 | - | - | - |
3 | - | 25,04 | - | 27,36 | 27,58 | 31,92 | - | - | - |
4 | 21,11 | 18,61 | 30,92 | 29,30 | 29,98 | 32,44 | - | - | - |
5 | 30,62 | 25,79 | - | 30,02 | 28,25 | 31,51 | - | - | - |
6 | 30,15 | 25,82 | >35 | 30,92 | 31,43 | 33,33 | - | - | - |
7 | 29,02 | 25,51 | >35 | 31,55 | 30,00 | 34,24 | - | - | - |
8 | >35 | 30,74 | - | 29,73 | 29,60 | 31,18 | - | - | - |
9 | 26,54 | 20,54 | 31,60 | 30,06 | 25,12 | 30,48 | - | - | - |
RNA from heart (HE), head kidney (HK) tissue and pylorus caeca (PC) tissue of Atlantic salmon with experienced symptoms of malabsorption and floating faeces in the fish pens. Real-time PCR Cp-values using ASCV field isolate specific primers in parallel with primers specific for PRV and PMCV, the causative agents for HSMI and CMS, respectively. >35 denotes samples with uncertain CP-values above 35, but with melting point temperature indicating a specific product. – denotes a negative sample.
*- Cp-values of parallel samples. Nd – not done due to availability of sample.