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. 2014 Sep 9;9(9):e107135. doi: 10.1371/journal.pone.0107135

Figure 3. NOX4 knockdown decreased TRPC1 and TRPC6 expression under both control and BMP4 treatment circumstances.

Figure 3

A: Western blot analysis of TRPC1 protein after knockout of NOX4 in rat distal PASMCs, of which the upper band is TRPC1, the following band is α-actin; NC and NOX4 siRNA group indicate negative control group and NOX4 knockout group respectively. B: results of knockout of NOX4 affecting TRPC1 protein in rat distal PASMCs. (X±S, n = 3), #indicates P<0.01 vs. Negative control group, the results have significant difference. C: Western blot analysis of BMP4 affecting TRPC1 in rat distal PASMCs after knockout of NOX4, of which the upper band is TRPC1, the following band is α-actin. NC+BMP4 group, NOX4 siRNA+BMP4 group indicate negative control group and NOX4-knockout with recombinant BMP4 treatment (for 24 hours) group, respectively. D: Results of BMP4 affecting TRPC1 expression in rat distal PASMCs after knockout of NOX4. (X±S, n = 3), #indicates P<0.01 vs. Negative control+BMP4 group, the results have significant difference. E: Western blot analysis of TRPC6 protein after knockout of NOX4 in rat distal PASMCs, of which the upper band is TRPC1, the following band is α-actin; NC and NOX4 siRNA group indicate negative control group and NOX4 knockout group respectively. F: results of knockout of NOX4 affecting TRPC1 protein in rat distal PASMCs. (X±S, n = 3), #indicates P<0.01 vs. Negative control group, the results have significant difference. G: Western blot analysis of BMP4 affecting TRPC6 in rat distal PASMCs after knockout of NOX4, of which the upper band is TRPC6, the following band is α-actin; NC+BMP4 NOX4 siRNA+BMP4 group indicate negative control group and NOX4-knockout with recombinant BMP4 treatment (for 24 hours) group, respectively. H: Results of BMP4 affecting TRPC6 expression in rat distal PASMCs after knockout of NOX4. (X±S, n = 3), #indicates P<0.01 vs. Negative control+BMP4 group, the results have significant difference.