Figure 1. Geographical distribution of strains isolated from the Central Tunisia study area.

Panel A shows the square delimited study area in the Governorate of Sidi Bouzid (in gray). Panel B represents the land satellite image of the study area showing the distribution of animal reservoir hosts from which the L. major strains were isolated. Isolates from P. obesus origin were noted P and colored in green, from M. shawi origin were noted Mer and colored in yellow, and from M. nivalis was noted M and colored in red. Spatial data related to the reservoir hosts of these strains were collected using the Global Positioning System (GPS). Satellite imagery: ArcGIS software.