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. 2014 Sep 9;9(9):e107128. doi: 10.1371/journal.pone.0107128

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© 2014 Deng et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A, Representative recording of 5-HT-evoked concentration-dependent contraction in fresh and cultured rat coronary arteries without endothelia (left panel). 5-HT induced markedly more contractions in cultured coronary arteries (full circles) than in fresh coronary arteries (open circles). B, U46619-induced contractions were comparable in fresh (open circles) and cultured coronary arteries (full circles). C, High K⁺ (60 mM)-induced contractions were comparable in fresh and cultured coronary arteries. D, Representative recording of 60 mM K⁺ solution before cumulative addition of CaCl₂ (left panel). CaCl₂-induced concentration-dependent contractions were not different in fresh (open circles) and cultured coronary arteries (full circles). The graph shows the mean ± SEM of 8–12 experiments on samples from different rats. **P<0.01 vs fresh coronary arteries.