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. 2014 Sep 9;9(9):e107128. doi: 10.1371/journal.pone.0107128

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© 2014 Deng et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A, RNA knockdown of Orai1 specifically reduced the expression of target proteins in coronary artery smooth muscle cells as assessed by western blotting. B, Orai1 downregulation significantly suppressed 5-HT-elicited Ca²⁺ influx in the presence of nifedipine 1 µM in coronary VSMCs. The graph shows the mean ± SEM of 5–8 experiments on samples from different rats. **P<0.01 vs control.