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. 2014 Sep 9;9(9):e107128. doi: 10.1371/journal.pone.0107128

Figure 5. Upregulation of signaling of the 5-HT receptor is involved in contractile hyperactivity in cultured coronary arteries.

Figure 5

A, 5-HT2A receptor, Orai1, STIM1, 5-HT2B receptor and L-type calcium channel mRNA expression levels in Fresh and Cultured coronary arteries. 5-HT2A receptor, Orai1, STIM1, 5-HT2B receptor and L-type calcium channel were expressed relative to GAPDH levels. Expression of the 5-HT2A receptor, STIM1 and Orai1 gene was augmented in cultured coronary arteries compared with fresh coronary arteries. The graph shows the means ± SEM of 4–6 experiments on samples from different rats. *P<0.05, **P<0.01 vs fresh coronary arteries. B, 5-HT2A receptor, Orai1, STIM1, 5-HT2B receptor and L-type calcium channel protein expression levels in Fresh and Cultured coronary arteries. 5-HT2A receptor, Orai1, STIM1, 5-HT2B receptor and L-type calcium channel were expressed relative to GAPDH levels. Expression of the 5-HT2A receptor, STIM1 and Orai1 protein was increased in cultured coronary arteries compared with fresh coronary arteries. The graph shows the means ± SEM of 4–6 experiments on samples from different rats. *P<0.05, **P<0.01 vs fresh coronary arteries.