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. 2014 Sep 9;9(9):e107369. doi: 10.1371/journal.pone.0107369

Figure 6. Tumorsphere cells derived from ER-positive breast cancer MCF7 cells express ER-α36-EGFR/HER2 positive regulatory loops.

Figure 6

A. Western blot analysis of the expression of ER-α36, ER-α66, EGFR, HER2 and ALDH1 in the monolayer MCF7 cells grown on attachment dishes (MCF7/P) and MCF7 tumorsphere cells grown on low-attachment dishes. B. Tumorsphere cells derived from MCF7TAM cells were treated with 5 µM of Broussoflavonol B (BB) or Lapatinib (LAP) for five days. Western blot analysis of expression levels of different proteins was performed. C. Tumorsphere cells derived from MCF7TAM cells were treated with 2.5 µM of Broussoflavonol B (BB), Lapatinib (LAP) or BB (2.5 µM) and LAP (2.5 µM) together for five days. Western blot analysis of expression levels of different proteins was performed.