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. 2014 Sep 9;9(9):e107369. doi: 10.1371/journal.pone.0107369

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© 2014 Yin et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A. Tumorsphere formation assay was used to assess the effects of TAM alone or together with Lapatinib (LAP) or Broussoflavonol B (BB) on ER-positive breast cancer stem/progenitor cells derived from parental MCF7 (MCF7/P) and tamoxifen resistant MCF7 cells (MCF7/TAM). The representative results are shown. B. The numbers of tumorspheres formed by MCF7/P and MCF7/TAM cells in the presence or absence of LAP and BB for 1^st and 2^nd generations. C. The number of cells from dissociated tumorspheres formed by MCF7/P and MCF7/TAM cells in the presence or absence of LAP and BB for 1^st and 2^nd generations. The columns represent the means of three experiments; bars, SE. * & #, P<0.05 for MCF/TAM cells treated with vehicle vs cells treated with tamoxifen and LAP or BB, respectively.