Table 1.
Dynamic light scattering results for uncoated and IL10-coated proticles and liposomes
| Mass ratio
|
Hydrodynamic diameter (nm) | Polydispersity index | Zeta potential (mV) | |
|---|---|---|---|---|
| ON:protamine | Mouse IL10 | |||
| aProticles | ||||
| 2:1 | 0 | 108.2 (±14) | 0.12 (±0.03) | −56.7 (±3.0) |
| 5E-5 | 121.0 (±6.6) | 0.10 (±0.02) | −34.7 (±5.4) | |
| 5E-3 | 122.3 (±6.8) | 0.09 (±0.01) | −38.0 (±1.2) | |
| 5E-2 | 118.8 (±3.9) | 0.10 (±0.01) | ||
| 1:1 | 0 | 114.5 (±12.8) | 0.10 (±0.03) | −29.5 (±5.5) |
| 5E-5 | 1,550.7 (±737.4) | 0.48 (±0.31) | −3.6 (±5.3) | |
| 5E-3 | 1,551.0 (±1,081.7) | 0.54 (±0.47) | −3.0 (±0.9) | |
| 5E-2 | 417.5 (±234.3) | 0.04 (±0.03) | ||
| bLiposomes | ||||
| Composition (molar lipid ratio) | ||||
| POPC:DSPE-PEG2000:cholesterol (3:0.3:2) | 150±1.02 (n=5) | 0.069±0.01 | −19.5±3.6 | |
| POPC:DSPE-PEG2000-NHS-IL10:cholesterol (3:0.3:2) | 162±0.6 (n=5) | 0.065±0.01 | −42.8±0.6 | |
Notes:
a
The composition of proticles is given as a mass ratio between the components, with a protamine concentration of 100 μg/mL. Results are given as mean values ± standard deviations from at least eight measurements (hydrodynamic diameter, polydispersity index) and four measurements (zeta potential).
b
Uncoated or IL10-coated liposomes were characterized by the same parameters.
Abbreviations: ON, oligonucleotides; IL10, interleukin 10; POPC, palmitoyl-oleoyl-phosphatidylcholine; DSPE, distearyl-phosphatidylethanolamine; PEG, polyethylene glycol; NHS, N-hydroxysuccinimide.