Figure 1.

DSas-6 and Ana2 Coassemble into Highly Ordered Tubules that Resemble the Centriole Cartwheel

(A–D) Mature primary spermatocytes expressing both GFP-DSas-6 and Ana2-GFP stained for the centriole marker GTU88^∗ (red), DNA (blue), and DSas-6 (green in A and C) or Ana2 (green in B and D). DSas-6 and Ana2 antibodies reveal the presence of large particles that often appear to be inserting between the paired centrioles. The arrow and arrowhead highlight a prematurely disengaged centriole pair, where one centriole has remained associated with the SAP (arrow), whereas the other has not (arrowhead).

(E) A mature primary spermatocyte expressing RFP-DSas-6 (red) and GFP-Ana2 (green) stained for DNA (blue). RFP-DSas-6 and GFP-Ana2 colocalize to the same cytoplasmic particles.

(F and G) Images show low (F) and high (G) magnification images of a SAP taken from an electron tomogram (see Movie S1 for entire tomogram). The SAPs are highly structured and consist of a well-defined central tubule (a “SAStubule”; viewed in cross section, black arrow, or in transverse section, black arrowheads) linked by spokes to an electron-dense outer ring (red arrow).

(H) An image from an electron tomogram showing a cross section through a spermatocyte centriole. Note the similarity between the central hub and spokes of the inner cartwheel (arrow in H) and the SAStubule and spokes of the SAP (black arrow in G). Scale bars, 10 μm (A), (B), and (E); 2 μm (C) and (D); and 100 nm (F)–(H). See also Figure S1 and Movies S1–S3.