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. 2010 Dec 14;19(6):913–919. doi: 10.1016/j.devcel.2010.11.010

Figure 2.

Figure 2

SAStubules Appear to Play a Role in Centriole Engagement

(A) Quantification of centriole number and conformation in mature primary spermatocytes expressing either GFP-DSas-6 (n = 141), Ana2-GFP (n = 141), or both GFP-DSas-6 and Ana2-GFP (n = 197). In ∼30% of spermatocytes coexpressing GFP-DSas-6 and Ana2-GFP, one or both centriole pairs prematurely disengaged, giving rise to centriole singlets rather than the normal doublets.

(B and C) Mature primary spermatocytes expressing either GFP-DSas-6 alone (B) or both GFP-DSas-6 and Ana2-GFP (C) stained for Asl (red), DNA (blue), and tubulin (green). In (C) the centrioles have prematurely separated to produce four singlets.

(D and E) Images taken from an electron tomogram showing the interaction between a SAP (outlined by dotted yellow lines) and the proximal ends of an engaged centriole pair (outlined by dotted red lines) (see Movie S4 for entire tomogram). Note how SAStubules extend away from the SAP to the centrioles (arrowheads).

(F and G) Images taken from electron tomograms that show the interaction between engaged centrioles in WT spermatocytes (see Movies S5 and S6 for entire tomograms). Note how tubules similar to the SAStubules link the engaged centrioles (arrowheads). Scale bars, 10 μm (B) and (C); and 100 nm (D)–(G). See also Movies S4–S6.