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. 2013 Aug;94(2):301–313. doi: 10.1189/jlb.0213093

Figure 4. DAP12 trafficks newly synthesized KIR2DS molecules to the cell surface.

Figure 4.

Surface expression of KIR2DS molecules was analyzed at 10 h and 16 h post-transfection of NKL cells with KIR and empty vector or DAP12. After the 10-h time-point, cells were left untreated or treated with BFA or CHX for 6 h and analyzed for KIR2DS surface expression. Flow cytometry analysis of surface expression was recorded for KIR2DS1 (A), KIR2DS2 (B), and KIR2DS4 (C). KIR2DS1 was used as a negative control for external staining for KIR2DS2 and KIR2DS4, whereas KIR2DS4 was used as the negative control for the KIR2DS1. Each experiment was performed with three independent biological samples resulting from three independent transfections/condition. Each experiment was repeated and analyzed in two independent experiments. Results (mean±sem) were analyzed using a one-way ANOVA, followed by Bonferroni's multiple comparison analysis (*P<0.05; **P<0.01; ***P<0.001). All comparisons to the control were statistically significant.