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. 2013 Aug;94(2):301–313. doi: 10.1189/jlb.0213093

Figure 5. DAP12 enhances the process of post-translational modifications of KIR2DS4.

Figure 5.

Expression of immature and mature isoforms of KIR2DS4 was analyzed post-transfection in the presence or absence of exogenous DAP12. (A) Representative Western blot analysis of KIR2DS4 isoform expression from whole lysates of tranfected NKL cells at the indicated time-points post-transfection in the presence or absence of exogenous DAP12 using a V5-specific antibody. β-Actin was used as a loading control. (B) Quantification of Western blots showing the change in percentage of receptor expressed as the mature isoform evaluated at each time-point compared with the values obtained 10 h post-transfection. The densitometry and quantification were performed on Western blot results from four independent experiments. The results were statistically analyzed by a two-way repeated-measures ANOVA followed by Bonferroni post-tests (*P<0.05; **P<0.01).