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. 2013 Aug;94(2):301–313. doi: 10.1189/jlb.0213093

Figure 6. DAP12 interacts with KIR2DS molecules early in the maturation process.

Figure 6.

(A) Western blot of immune complexes obtained following immunoprecipitation of lysates from NKL cells cotransfected with constructs encoding KIR2DS1 and HA-DAP12 with a DAP12-specific antibody. The membrane was probed using antibodies specific for the V5 (KIR) or HA (DAP12) epitope. (B) Western blot of NKL lysates using a V5-specific antibody following expression of KIR2DS molecules, with or without exogenous DAP12. The membrane was also analyzed for DAP12 expression. β-Actin served as the internal loading control. (C) Densitometry ratios calculated from the Western blot in B showing a ratio of mature KIR expression (upper part of top band in B) to total KIR expression. (D) Densitometry ratios calculated from the Western blot in B showing a ratio of immature KIR expression (lower part of top band in B) to total KIR expression. The Western blot and subsequent densitometry data represent the results of three independent experiments.