Table 2.
Functional recombinant protein is present in the pre-induction phases of P. pastoris cultures when cultivated in bioreactors, but not in shake-flasks
| Protein | Bioreactor | Shake-flask | ||
|---|---|---|---|---|
| Pre-induction expression in glycerol-grown cells? | Pre-induction expression in glucose-grown cells? | Pre-induction expression in glycerol-grown cells? | Pre-induction expression in glucose-grown cells? | |
| Integral membrane proteins | ||||
| hA2aR | Y (Figures 1 and 2) | Y (Figure 4) | N [18] | – |
| CD81 | – | – | N (Additional file 1: Figure S1) | – |
| CD82 | – | – | N (Additional file 1: Figure S1) | N (Additional file 1: Figure S1) |
| Claudin-1 | – | – | N (Additional file 1: Figure S1) | N (Additional file 1: Figure S1) |
| Peripheral membrane protein | ||||
| hCGRP-RCP-GFP | Y (Table S1) | – | – | – |
| Soluble proteins | ||||
| GFP | Y (Figures 3 and 6A) | Y (Figures 5 and 6B) | N (Additional file 1: Table S2) | N (Additional file 1: Table S2) |
| HRP | – | – | N (Additional file 1: Table S3) | – |
The presence (Y) or absence (N) of recombinant protein was determined in bioreactor and shake-flask cultivations for glycerol- and glucose-grown cells as indicated; the evidence supporting these designations is given in parentheses and refers to figures within the text or in supplementary files; “–” indicates that the experiment was not done.