Fig. 2.

Poly-GA forms p62-positive aggregates in neurons. a Immunofluorescence of primary hippocampal neurons transduced with GA₁₄₉-GFP or GFP control lentivirus at day 6 in vitro for 15 days (DIV6 + 15). Immunostaining for p62 and the dendritic marker protein MAP2. DAPI was used a nuclear marker. Poly-GA forms p62-positive inclusions (arrow) in the soma and dendrites. Scale bar 15 µm. b Immunoblotting of primary cortical neurons transduced with GA₁₄₉-myc or GFP control lentivirus (DIV6 + 17) with the indicated antibodies. Poly-GA aggregates are stuck at the top of the gel (arrow). GA₁₄₉-myc induces upregulation of p62, but levels of TDP-43 and the synaptic marker protein PSD-95 are not affected. Three separate transductions are shown. c Filter trap assay of primary cortical neurons transduced with GA₁₄₉-myc or GFP (DIV6 + 17). Poly-GA aggregates are detected in the serial dilution of homogenates using anti-GA