Fig. 7.

Unc119 co-aggregates with poly-GA, but not with TDP-43 in patients with C9orf72 mutation. Double immunofluorescence analysis of Unc119 with poly-GA or phosphorylated TDP-43 (pTDP-43) in C9orf72 mutation cases C9-1, C9-2 and C9-3. a In the superior frontal gyrus (SFG), a subset of poly-GA-positive neuronal cytoplasmic inclusions also contains Unc119. Redistribution of Unc119 compared to GA-negative cells can be seen in a fraction of co-aggregating cells (white arrows). b In the cerebellar granular cell layer (CBL) abundant cytoplasmic poly-GA inclusions are only rarely positive for Unc119 (white arrows). c As shown for the superior frontal gyrus, Unc119 (white arrow) and pTDP (red arrow) are not co-localized in the same cytoplasmic inclusions. Scale bars represent 10 μm for overviews and 5 µm for the close-up in the second column