Table 1.
Poly-GA interacting proteins identified by quantitative MS analysis
| Gene names | Protein IDs | −log10(p) | log2(GA/ctrl) | Protein names |
|---|---|---|---|---|
| Sqstm1 | O08623-2; O08623; O08623-3 | 3.37 | 7.25 | p62/Sequestosome-1 |
| Rad23a; Rad23b | Q4KMA2; Q5XFX7 | 1.79 | 5.47 | UV excision repair protein RAD23 homolog A and B |
| Ubqln2; Ubqln4 | D4AA63; D4A3P1 | 2.77 | 4.57 | Ubiquilin 2; Ubiquilin 4 |
| Sdcbp | Q9JI92 | 2.43 | 4.52 | Syntenin-1 |
| Ubb; Ubc; Uba52; Rps27a; LOC100360645 | G3V9Z2; P0CG51; Q63429; F1LML2; Q6P7R7; P62986; Q6PED0; P62982; F1M516 | 3.04 | 4.51 | Polyubiquitin-B; Ubiquitin; Polyubiquitin-C; Ubiquitin; Ubiquitin-related; Ubiquitin-60S ribosomal protein L40; Ubiquitin; 60S ribosomal protein L40; Ubiquitin-40S ribosomal protein S27a; Ubiquitin; 40S ribosomal protein S27a |
| Klhdc10 | Q5U3Y0; D3ZUK9 | 2.10 | 4.46 | Kelch domain-containing protein 10 |
| Bag6 | Q6MG49; Q6MG49-2 | 2.81 | 3.71 | Large proline-rich protein BAG6 |
| Psmb6 | P28073 | 2.95 | 3.65 | Proteasome subunit beta type-6 |
| Ubqln1 | F1M971; Q9JJP9 | 1.42 | 3.47 | Ubiquilin 1 |
| Dbn1 | Q07266; Q07266-2; C6L8E0 | 1.91 | 3.36 | Drebrin |
| Myh10; Myh14 | Q9JLT0; G3V9Y1; F1LNF0 | 1.70 | 3.28 | Myosin-10; Myosin-14 |
| Efhd1; Efhd2 | Q4FZY0; D4A9T5 | 1.56 | 3.25 | EF-hand domain-containing protein D1; EF-hand domain-containing protein D1 |
| Acat2 | Q5XI22; F1LS48 | 1.34 | 3.21 | Acetyl-CoA acetyltransferase, cytosolic |
| Mlf2 | D3ZPN3 | 2.32 | 3.13 | Myeloid leukemia factor 2 |
| Psmc6 | G3V6W6 | 1.70 | 3.12 | Proteasome (prosome, macropain) 26S subunit, ATPase, 6 |
| Psmb4 | D4A640; P34067; G3V8U9 | 3.44 | 3.10 | Proteasome subunit beta type-4 |
| Psmb5 | G3V7Q6; P28075 | 1.97 | 2.98 | proteasome (prosome, macropain) subunit, beta type, 5 |
| Unc119; Unc119b | Q62885; F1LZN2; D3ZY58 | 1.97 | 2.90 | Protein unc-119 homolog A and B |
| Myo5b; Myo5c | F1M111; F1M3R4; P70569 | 1.68 | 2.85 | Myosin VB/C |
| Psmd13 | B0BN93 | 3.01 | 2.68 | 26S proteasome non-ATPase regulatory subunit 13 |
Protein enrichment in GA149-GFP immunoprecipitates compared to GFP control determined by quantitative mass spectrometry. Listed are the NCBI gene names, the UniProt identifier, logarithmic p value and enrichment factor. Statistical analysis using t test at a false discovery rate of 2 % and S0 of 2 [50]. Proteins with shared peptides are clustered in groups. Graphical representation is depicted in Fig. 4a