Figure 6.

Comparison of uptake of FAM-InsP₅ (5) and InsP₆. A) H1299 cells were incubated with 5 or InsP₆ (20 μm) for 3 h at 37 °C. After washing of the cells five times with PBS, InsP₆ was extracted as previously described[8] and analysed by MDD-HPLC. For analysis of the concentration of 5, washed cells were extracted as described in the Experimental Section, and the concentration of 5 was analysed with a fluorescence reader, on the basis of a standard curve for 5 at excitation 490 nm and emission 535 nm. B) Analysis of fluorescence of the washing fractions of cells treated with 5; see (A).