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. Author manuscript; available in PMC: 2015 Sep 8.
Published in final edited form as: Dev Cell. 2014 Sep 8;30(5):610–624. doi: 10.1016/j.devcel.2014.07.014

Figure 5. Exomer function correlates with its ability to be recruited to membranes in vitro.

Figure 5

Plasmids expressing Chs6-GFP and Bch1-mCherry constructs were introduced into a chs6Δbch1Δbud7Δ strain. Bch1 interface mutations were introduced together with either wild-type Chs6 or the Chs6 site 1+2 mutant. Serial dilutions were spotted on the indicated media and imaged after 2 days at 30°C. Calcofluor was used at 100 μg/ml. Cells with disrupted Chs3 trafficking grow in the presence of calcofluor white.