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. Author manuscript; available in PMC: 2015 Sep 8.
Published in final edited form as: Dev Cell. 2014 Sep 8;30(5):610–624. doi: 10.1016/j.devcel.2014.07.014

Figure 7. Characterization of exomer membrane remodeling activity.

Figure 7

A) Vesiculation reactions were performed as in Fig. 6A,B, but analyzed by DLS. Samples were separated into two different graphs for simplicity; they constitute an example of a single experiment.

B) DLS analysis of reactions similar to Fig. 6J.

C) Representative negative stain transmission electron micrographs of vesiculation reactions. Scale bar, 500 nm.

D) Structural model of the exomer/Arf1 complex generated by normal model analysis. The Chs5 hinge would allow the complex to bind to a highly curved membrane.

E) Pelleting of sucrose-laden 30 nm liposomes demonstrates exomer complexes bind to highly curved membranes.

F) Model for the dual functions of exomer in cargo sorting and membrane remodeling. Due to differences in membrane-remodeling capacity, we envision distinct exomer complexes may be responsible for cargo sorting (bound to cargo within the nascent vesicle) and vesicle fission (positioned at the constrained neck).

See also Figure S5.