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. Author manuscript; available in PMC: 2015 Sep 8.
Published in final edited form as: Cancer Cell. 2014 Sep 8;26(3):358–373. doi: 10.1016/j.ccr.2014.07.022

Figure 4. FBXO11 blocked SNAIL-induced EMT and metastasis in 4T1 breast cancer cell line.

Figure 4

(A) Phase contrast and IF images for 4T1 cells transduced with indicated lentiviruses. SNAIL-induced strong EMT program in 4T1 cells with downregulation of E-cadherin and upregulation of N-cadherin expression, while FBXO11 blocked SNAIL-induced EMT. Scale bars, 25µm. (B) qRT-PCR analysis of EMT marker mRNAs in the indicated 4T1 cell lines. Results were normalized to GAPDH mRNA level. Data represent average ± SEM, *p < 0.05 by Student's t test. (C) GSEA analysis demonstrated FBXO11 expression inhibited SNAIL induced EMT gene signature in 4T1 cells. (D) Heatmap was generated using hierarchical clustering. The 1400 gene probes used for clustering were those showing >2 folds expression changes in SNAIL overexpressing cells. (E) 105 cells was orthotopically injected into Balb/c mice (n = 10). Mice were sacrificed 3 weeks later. Representative lung metastasis nodule images were presented. (F) Numbers of lung metastasis lesions of mice injected with the indicated 4T1 cell lines. **p < 0.01 by Mann–Whitney U test. See also Figure S4.