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. Author manuscript; available in PMC: 2015 Sep 8.
Published in final edited form as: Cancer Cell. 2014 Sep 8;26(3):358–373. doi: 10.1016/j.ccr.2014.07.022

Figure 5. Knockdown of endogenous FBXO11 promotes breast cancer metastasis.

Figure 5

(A) Real-time PCR analysis of Fbxo11, Cdh1 and Vimentin expression in the parental and control 4T1 cell line, and two Fbxo11-KD cell lines. Data represent mean ± SD. **: p < 0.01 by two-tailed Student’s t test. (B) Western blot analysis of Snail, E-cadherin and Vimentin expression in the indicated 4T1 cell lines. (C) Phase contrast images and immunofluorescent images of EMT markers in the indicated 4T1 cell lines. Scale bars, 25µm. (D) 105 tumor cells from various 4T1 cell lines were orthotopically injected into Balb/c mice and primary tumors were removed 10 days later. Lung metastasis nodules were counted after sacrificing the mice at 38 days post injection. **p < 0.01 by Mann–Whitney U test. (E) Representative lung metastasis nodule images were presented. See also Figure S5.