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. Author manuscript; available in PMC: 2015 Sep 8.
Published in final edited form as: Cancer Cell. 2014 Sep 8;26(3):358–373. doi: 10.1016/j.ccr.2014.07.022

Figure 8. SNAIL protein expression level is positively correlated with lymph node invasion and negatively correlated with FBXO11 expression level in breast cancer patients.

Figure 8

(A) Correlation study of SNAIL expression level with lymph node invasion in 136 breast tumor specimens. SNAIL-lo: SNAIL IHC staining lower than median; SNAIL-hi: SNAIL IHC staining higher than median; LN-: without lymph node invasion; LN+: with lymph node invasion. χ2=4.9, p = 0.026 by chi-square test. (B) Kaplan-Meier plots of distant relapse-free survival of patients, stratified by expression of PKD1. Data obtained from the Kaplan-Meier plotter database (Gyorffy et al., 2010). (C) Correlation study of activated PKD1 (pY95-PKD1) and S11-SNAIL in a breast tumor tissue microarray (US Biomax BC081120), χ2=10.0, p = 0.0016 by chi-square test. (D) Representative IHC images of pY95-PKD1 and pS11 -SNAIL in breast tumors. Scale bars, 100µm. (E) Correlation study of SNAIL and FBXO11 in the same breast tumor tissue microarray. χ2=7.49, p = 0.0062 by chi-square test. (F) Representative IHC images of SNAIL and FBXO11 in breast tumors. Scale bars: 100µm. (G) Schematic model for PKD1-dependent SNAIL protein ubiquitylation and degradation by FBXO11. SNAIL protein is first phosphorylated by PKD1 kinase at Serine-11 residue before it can be recognized and ubiquitylated by SCF-FBXO11 E3 ligase complex. Poly-ubiquitylated SNAIL protein is then degraded through 26S proteosomal degradation pathway.