Fig. 7.

Impact of the GtfCH GTs on the abundance of the glycoforms of the [192–211] peptide. The glycoforms of the [192–211] peptide (Table II) issued from Srr1 synthesized in H36BSrtA* (gray bars) and H36BSrtA*ΔgtfCH (white bars) strains were quantified by calculating the area of each parent ions computed from XIC and normalized (see experimental procedures). LC-MS/MS experiments were performed in triplicates and standard deviation (S.D.) is reported for each quantified glycoform. All Hex containing glycoforms were absent in Srr1 produced in ΔgtfCH background.