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. 2014 May 22;13(9):2213–2232. doi: 10.1074/mcp.M113.037085

Fig. 6.

Fig. 6.

Quantitative RT-PCR of mRNA transcripts of glycosyltransferase genes and scatterplot analysis of glyco-gene expression (MGAT3 and ST6GAL1) with corresponding N-glycan structures. Gene expression levels of glycosyltransferase mRNA transcripts (n = 17) analyzed in two non-cancerous human ovarian surface epithelial cells (HOSE 6.3 and HOSE 17.1) and four ovarian cancer cell lines (SKOV 3, OVCAR 3, A2780, and IGROV 1). A, Normalized (ΔCq) and clustered “glyco gene” expression (row) among tested cell lines (column) visualized as heatmap. Dendrogram (row) shows clusters of correlating expression. Key at the right side indicates level of expression of transcripts from high (black) to low (white). Level of significant differences in transcript levels are indicated by asterisk (• p < 0.1; * p < 0.05; ** p < 0.001). Gene expression profiles of MGAT3, ST6GAL1 and ST3GAL5 show positive correlation with resulting glycan phenotype as illustrated by B, scatterplots of MGAT3, ST6GAL1, and ST3GAL5 “glyco gene” expression (abscissa) and their corresponding relative MS ion intensities of bisecting GlcNAc, α 2–6 and α 2–3 sialylated N-glycan structures (ordinate). Scatterplot data points circled in dashes represent two non-cancerous human ovarian surface epithelial cells and data points circled in dots represent four ovarian cancer cell lines used in this study. Data represents the mean of three technical replicates.