Table II. Established target gene primers and reference genes. Comprehensive list of primers used for quantitative RT-qPCR and parameters providing efficiency (E) and correlation coefficient (R²) for each primer pair on target and reference genes applied in this study. Reference genes were established by Jacob et al. (2013) (44).

Symbol	Gene name	Accession number	Chromosomal location	Forward primer 5′-3′	Reverse primer 5′-3′	E in %	R2
MGAT3	mannosyl (β-1,4-)-glycoprotein β-1,4-N-acetylglucosaminyl transferase	NM_002409.4	22q13.1	GGGATGAAGATGAGACGCTACAAG	AGGACAGGGTCTTGAAGAAGTGC	114.3	0.985
ST6GAL1	β-galactosamide α-2,6-sialyltransferase 1	NM_173217.2	3q27-q28b	CCATCCTCTGGGATGCTTGGTATC	ACGTCAGTCTTGCGCTTGGATG	102.9	0.991
ST3GAL1	β-galactoside α-2,3-sialyltransferase 1	NM_173344.2	8q24.22	AGTCACGACTTTGTCCTCAGGATG	TGGTCTTGGTCCCAACATCAGC	83.3	0.981
ST3GAL2	β-galactoside α-2,3-sialyltransferase 2	NM_006927.3	16q22.1	GCATGTGTGTGATGAGGTGAACG	TTCTCCCAGTAGTGGTGCCAGTTG	102.6	0.999
ST3GAL3	β-galactoside α-2,3-sialyltransferase 3	NM_174963	1p34.1	AGTGGCAGGACTTTAAGTGGTTG	AGAAGCCATCCGATGCACTCAC	91.4	0.963
ST3GAL4	β-galactoside α-2,3-sialyltransferase 4	NM_006278.2	11q24.2	CAGCCACGGAAGATTAAGCAGAAG	GCAATGTGCACCAAGTCACAGAG	111.8	0.999
ST3GAL5	β-galactoside α-2,3-sialyltransferase 5	NM_001042437.1	2p11.2	TGTGGACCCTGACCATGTAAAGAG	TGGCAAACTTGGGACGACATTCC	103.6	0.995
FUT2	α1–2 fucosyltransferase 2	NM_000511.5	19q13.3	TCACCGATGCTGGAAGGGTTTC	AACGACCAGCATGGCTTCTCTC	118.2	0.992
FUT3	α1–3/4 fucosyltransferase 3	NM_001097640.1	19p13.3	TCAGACAGGTCCAAGTTCAAGCC	TTACAGTCGATCCCACCTGTACCC	107.8	0.997
FUT4	α1–3 fucosyltransferase 4	NM_002033.3	11q21	TTTACCGAGGAAGGAGCCAAGG	GCTGGTTCTGCCACTGCTATTG	100.0	0.999
FUT5	α1–3 fucosyltransferase 5	NM_002034.2	19p13.3	TGCATCACTATGGGTGTGACCTC	TGAGGCATCGCAACACATCCAC	120.5	0.952
FUT8	α1–6 fucosyltransferase 8	NM_178155	14q24.3	GGCCTGTAAGTGAGACATGCAC	TTTGTCCTTCACTTCACCTGACC	89.4	0.998
FUT9	α1–3 fucosyltransferase 9	NM_006581.3	6q16	TGGCCTCATTAGCCACCTTCAG	TGGACAAGGATGGCATCTTCAGG	93.1	0.987
B4GALNT1	beta-1,4-N-acetyl-galactosaminyl transferase 1	NM_001478.3	12q13.3	GAGCCTTCAGGCAGCTTCT	CCTAGGGAGGCAGTCAGGTT	98.1	0.910
B4GALNT3	beta-1,4-N-acetyl-galactosaminyl transferase 3	NM_173593.3	12p13.33	TGGCATAGACCTCGTGAAGGAC	ACAGTGCTTCCGAATGGCATC	100.4	0.998
B3GALNT1	beta-1,3-N-acetyl-galactosaminyltransferase 1	NM_003781.3	3q25	TGCTCTATCACGTGGTGCTCTC	ACGCGAGCCGAAGGTTCTTTAC	116.8	0.997
B3GALNT2	beta-1,3-N-acetyl-galactosaminyltransferase 2	NM_152490.2	1q42.3	TGCCTTACTGAAGGAGGAAAGCAG	AGCTCGTTGTTTCCACAGTCCATC	106.3	0.986
HSPCB	Heat shock protein 90 kDa alpha (cytosolic)	NM_007355	6p12	TCTGGGTATCGGAAAGCAAGCC	GTGCACTTCCTCAGGCATCTTG	103.1	0.998
YWHAZ	Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide	NM_003406	8q23.1	ACTTTTGGTACATTGTGGCTTCAA	CCGCCAGGACAAACCAGTAT	101.2	0.998
SDHA	Succinate dehydrogenase complex, subunit A	NM_004168	5p15	TGGGAACAAGAGGGCATCTG	CCACCACTGCATCAAATTCATG	105.6	0.994