Fig. 1.

Expression of the human SNX-BAR proteins in L. tarentolae cell free lysate. A, Ribbon representation of a representative crystal structure of the SNX9 dimer (PDB:2RAI), viewed from the convex side (top) and the lateral side (bottom). One SNX9 subunit is shown in red/orange/yellow, the other in green/cyan/blue. Red/blue indicates the two PX (Phox) domains, orange/cyan the two yoke domains and yellow/green the two BAR (Bin, Amphiphysin, Rvs) domains. Dimerization of the BAR domains leads to a rigid banana-shaped structure. B, Domain structures of the 12 members of the sorting nexin (SNX) family analyzed here. All proteins possess a PX domain represented as a blue rectangle. Except for SNX3, all other proteins also contain a C-terminal BAR domain indicated in red. A Src Homology 3 (SH3) domain (orange circle) is also present in SNX9 and SNX33. C, SDS-Page analysis of the LTE expressed SNX-BAR domain proteins. Each protein was labeled with a N-terminal GFP tag or a Cherry-myc tag. Proteins were separated on SDS-PAGE gel (4–12% Tris-glycine) and visualized by in gel fluorescence scanning.