Fig. 4.

Biological pathway enrichment of identified proteins in the embryos of honey bee workers (A.m.ligustica). A, Comparison of enriched biological pathways of the identified proteins in the embryo of the honey bee workers (A.m.ligustica) aged 24h, 48h, and 72h. Significant enriched pathways are analyzed by KEGG Orthology-Based Annotation System (KOBAS, http://kobas.cbi.pku.edu.cn) (37). The pathway enrichment is conducted by a hypergeometric statistic test. The Benjamini and Hochberg FDR correction is used to correct the probability values, and only the corrected p < 0.05 is considered as statistically significant enriched pathways. B, Representative enriched pathway map from KOBAS. Green labeled boxes are the protein references of honey bee (A.m.ligustica) annotated to the KEGG PATHWAY database, highlighted red ones indicate the protein entries mapped to the significantly enriched pathway.