Fig. 8.

Test of the 16 differentially expressed (fold change ≥2 and p < 0. 05) proteins at the mRNA level by quantitative real time PCR analysis. The mRNA expression is normalized with the reference gene (GAPDH). The color bars represent the relative expression values of mRNA and protein in different aged embryos. (a) is significantly higher than (b) and (c), (b) is significantly higher than (c). Error bar is standard deviation. Abbreviated protein names indicate different proteins as in supplemental Table S1.