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. 2014 Jun 3;13(9):2306–2320. doi: 10.1074/mcp.M114.037846

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© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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Fig. 9. — Western blotting analysis of nucleoside diphosphate kinase (NDPK), lysyl-tRNA synthetase (LysRS), elongation factor 1 alpha (eEF1A), eukaryotic translation initiation factor 5A (eIF-5A), 40S ribosomal protein S11 (RPS11). The protein samples from worker embryos (A.m.ligustica) are subjected to SDS-PAGE followed by Western blotting analysis. NDPK, LysRS, eEF1A, eIF-5A, and RPS11 are detected using the corresponding polyclonal antibody. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is used as reference control. A, The Western blotting bands of NDPK, LysRS, eEF1A, eIF-5A, RPS11, and GAPDH. B, The relative expression values of NDPK, LysRS, eEF1A, eIF-5A, and RPS11 in the three aged honey bee (A.m.ligustica) embryos (normalized by GAPDH). (a) is significantly higher than (b) and (c), (b) is significantly higher than (c). Error bar is standard deviation.