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. 2014 Jun 10;13(9):2354–2370. doi: 10.1074/mcp.M114.038315

Fig. 2.

Fig. 2.

Alterative O-glycan structure identified using ZIC-HILIC enrichment with A. baumannii strains. ITMS-CID fragmentation enabled the identification of A. baumannii glycopeptides containing alterative glycoforms within all strains examined including: A, A tetramer glycan composed of HexNAc2-Hex-NulOAc within A. baumannii SDF (48SAGDQAASDIATATDNASAKIDAATDHAADATAK81 of B0VLI0_ACIBS), B, A trisaccharide composed of HexNAc-dHexNAc-NulOCH3 within A. baumannii 1441 C1 (48SAGDQAASDIATATDNASAK67 of A7FB63_ACIBT), C, A disaccharide composed of HexNAc2 within A. baumannii SDF (48SAGDQAASDIATATDNASAK67 of B0VLI0_ACIBS), D, A pentamer glycan within A. baumannii 19606 composed of HexNAc-Hex2-HexNAc-GlcNAc3NAcA (48SAGDQAASDIATATDNASAK67 of D0CEI7_ACIBA), E, A pentamer glycan within A. baumannii 19606 and 17978 composed of HexNAc-Hex2-HexNAc-GlcNAc3NAcA4OAcCH3 (48SAGDQAASDIATATDNASAK67 of A7FB63_ACIBT), F, The known pentamer glycan of A. baumannii ATCC 17978 where a Hex has been exchanged for a HexNAc residue compared with the previously reported glycan (14) (48SAGDQAASDIATATDNASAK67 of A7FB63_ACIBT), G, The known pentamer glycan of A. baumannii ATCC 17978 where the linking HexNAc residue has been exchanged for the unique 386.11 Da residues compared with the previously reported glycan (14) (48SAGDQAASDIATATDNASAK67 of A7FB63_ACIBT), I, A dimer of the trisaccharide O-glycan of A. baumannii 1441 C1 (19NDGMHEASDPATSHDMNK36 of A7FB95_ACIBT), and J, A trimer of the trisaccharide O-glycan of A. baumannii 1441 C1 (302AKPASTPAVK311 of A3M265_ACIBT).