Table I. List of identified propionylproteins in T. thermophilus HB8.
| ORF | Definition | Propionylation sites |
|---|---|---|
| Metabolism | ||
| Energy production and conversion | ||
| TTHA0027 | Probable potassium channel, β subunit (oxidoreductase) | K31, K252 |
| TTHA0089 | NADH-quinone oxidoreductase chain 1 | K58 |
| TTHA0185 | Pyruvate dehydrogenase complex, E1 component | K323, K429 |
| TTHA0206 | Nicotinamide nucleotide transhydrogenase, α subunit 1 | K235 |
| TTHA0229 | 2-oxoisovalerate dehydrogenase, E1 α subunit | K294 |
| TTHA0230 | 2-oxoisovalerate dehydrogenase, E1 β subunit | K33, K181 |
| TTHA0232 | Pyruvate dehydrogenase complex, E2 component | K265, K274 |
| TTHA0233 | Pyruvate dehydrogenase complex, E3 component | K76, K82, K121, K170, K232, K240, K280, K284 |
| TTHA0278 | ATP-dependent phosphoenolpyruvate carboxykinase | K512 |
| TTHA0287 | 2-oxoglutarate dehydrogenase E3 component | K71, K220, K239 |
| TTHA0288 | 2-oxoglutarate dehydrogenase E2 component | K131, K219, K227, K393 |
| TTHA0466 | Alcohol dehydrogenase | K225 |
| TTHA0506 | Malate synthase | K144, K329 |
| TTHA0520 | NAD-dependent malic enzyme (malate dehydrogenase) | K10 |
| TTHA0537 | Succinyl-CoA synthetase α chain | K42, K112, K143 |
| TTHA0538 | Succinyl-CoA synthetase β chain | K45, K73, K84, K298, K311, K334, K362 |
| TTHA0558 | Fumarate hydratase class II (EC 4.2.1.2) | K121, K420 |
| TTHA0968 | Phenylacetic acid degradation protein PaaZ | K132, K305 |
| TTHA0996 | Succinate-semialdehyde dehydrogenase | K82 |
| TTHA1146 | Electron transfer flavoprotein, α subunit | K17 |
| TTHA1272 | V-type ATP synthase subunit B | K86 |
| TTHA1273 | V-type ATP synthase subunit A | K97 |
| TTHA1275 | V-type ATP synthase subunit | K271 |
| TTHA1279 | V-type ATP synthase, subunit (VAPC-THERM) | K31, K67, K97 |
| TTHA1343 | Citrate synthase | K273, K300 |
| TTHA1378 | Homoisocitrate dehydrogenase | K76 |
| TTHA1454 | Succinate dehydrogenase, flavoprotein subunit | K125, K557, K563, K573 |
| TTHA1535 | Isocitrate dehydrogenase | K46, K97, K105, K185, K196, K203, K343, K357, K368, K421, K457 |
| TTHA1578 | 1-pyrroline-5-carboxylate dehydrogenase | K73 |
| TTHA1836 | Isocitrate lyase | K190, K324, K387 |
| TTHA1965 | Inorganic pyrophosphatase | K160 |
| TTHB240 | 5-carboxy-2-hydroxymuconate semialdehyde dehydrogenase | K8 |
| Amino acid transport and metabolism | ||
| TTHA0046 | Aspartate aminotransferase | K250 |
| TTHA0216 | Alanine dehydrogenase | K73 |
| TTHA0457 | 3-phosphoshikimate 1-carboxyvinyltransferase | K22 |
| TTHA0545 | Aspartate-semialdehyde dehydrogenase | K274 |
| TTHA0582 | Aspartate aminotransferase, subgroup IV | K299, K317 |
| TTHA0722 | Histidinol dehydrogenase | K59 |
| TTHA1210 | 2-isopropylmalate synthase (LeuA) | K115, K357, K415 |
| TTHA1211 | Probable ketol-acid reductoisomerase (IlvC) | K55, K120 |
| TTHA1212 | Acetolactate synthase, small subunit (ilvN) | K62 |
| TTHA1496 | Arginase | K176 |
| TTHA1524 | Serine hydroxymethyltransferase | K245, K326 |
| TTHA1698 | Carboxypeptidase G2 | K102 |
| TTHA1755 | Acetylornithine/acetyl-lysine aminotransferase | K346, K356 |
| TTHA1781 | GTPase ObgEa | K30, K147 |
| TTHA1928 | γ-Glutamyl phosphate reductase | K75, K232, K317 |
| Carbohydrate transport and metabolism | ||
| TTHA0002 | Enolase (2-phosphoglycerate dehydratase) | K61 |
| TTHA0003 | Pyruvate kinase | K74 |
| TTHA0106 | Ribulose-phosphate 3-epimerase | K72 |
| TTHA0108 | Transketolase | K25, K347 |
| TTHA0116 | Phosphonopyruvate decarboxylase | K192, K217, K300 |
| TTHA0299 | Glucokinase | K21, K33 |
| TTHA0481 | Oligo-1,6-glucosidase | K435 |
| TTHA0650 | Putative phosphoglucomutase/phosphomannomutase | K127 |
| TTHA0905 | Glyceraldehyde 3-phosphate dehydrogenase | K75, K112, K190, K221, K246, K267, K294, K297 |
| TTHA0906 | Phosphoglycerate kinase | K10, K122, K244 |
| TTHA0980 | Bifunctional fructose 1,6-bisphosphate aldolase/phosphatasea | K84, K233 |
| TTHA1299 | Ribose 5-phosphate isomerase | K67 |
| TTHA1446 | Fructose-1,6-bisphosphatase, class II | K31 |
| TTHA1773 | Fructose-1,6-bisphosphate aldolase | K148, K240, K241, K273, K279 |
| Coenzyme transport and metabolism | ||
| TTHA0341 | Molybdopterin biosynthesis enzyme, MoaB | K133 |
| TTHA0345 | Porphobilinogen deaminase | K26 |
| TTHA0617 | Nicotinate phosphoribosyltransferase | K428 |
| TTHA0634 | Magnesium chelatase related protein | K110 |
| TTHA0966 | Phenylacetyl-CoA ligase | K439 |
| TTHA1120 | Methylene-THF dehydrogenase (cyclohydrolase) | K169 |
| TTHA1642 | S-adenosylmethionine synthetase | K37 |
| TTHA1738 | 3-oxoadipate enol-lactonasea | K100, K135 |
| TTHA1789 | Molybdenum cofactor biosynthesis protein C | K19 |
| TTHB053 | Precorrin-6Y C5,15-methyltransferase [decarboxylating] | K85, K111, K186, K231 |
| TTHB054 | Precorrin-2 methylase | K170 |
| TTHB055 | Precorrin-4 C11-methyltransferase | K57 |
| TTHB057 | Cobalamin biosynthesis protein CbiG | K91, K274, K336 |
| Lipid transport and metabolism | ||
| TTHA0413 | 3-oxoacyl-[acyl carrier protein] synthase II | K245 |
| TTHA0415 | 3-oxoacyl-[acyl carrier protein] reductase | K38 |
| TTHA0559 | Acetyl coenzyme A acetyltransferase (thiolase) | K16, K188, K212 |
| TTHA0750 | 3-oxoacyl-[acyl carrier protein] reductase | K95, K196 |
| TTHA0890 | Putative 3-hydroxyacyl-CoA dehydrogenase | K304, K467 |
| TTHA0892 | Acyl-CoA dehydrogenase | K221, K266 |
| TTHA0987 | β-Ketoadipyl CoA thiolase | K211, K214 |
| TTHA1124 | Acetyl-CoA carboxylase, biotin carboxyl carrier protein | K111, K143 |
| TTHA1248 | Acetyl-coenzyme A synthetase | K368, K612, K629 |
| TTHA1262 | 3-hydroxybutyryl-CoA dehydrogenase | K58, K285 |
| TTHA1434 | 3-hydroxybutyryl-CoA dehydratase | K81, K87, K142 |
| TTHA1768 | Acetyl-CoA carboxylase carboxyl transferase, β subunit | K87 |
| TTHB019 | MaoC-related acyl dehydratase | K114 |
| Nucleotide transport and metabolism | ||
| TTHA0075 | Ribonucleoside-diphosphate reductase | K51, K200, K2024, K2060 |
| TTHA0188 | Nucleoside diphosphate kinase | K55, K94 |
| TTHA0432 | IMP dehydrogenase/GMP reductase | K411, K417 |
| TTHA1466 | CTP synthase | K462 |
| TTHA1552 | GMP synthase | K102, K359 |
| TTHA1614 | Adenine phosphoribosyltransferase | K176 |
| TTHA1671 | Adenylate kinase | K150 |
| TTHA1742 | Orotate phosphoribosyltransferase | K88, K98 |
| TTHB209 | Ribonucleoside-diphosphate reductase, α subunit | K38 |
| Inorganic ion transport and metabolism | ||
| TTHA0045 | Probable potassium uptake protein TrkA | K372 |
| TTHA0122 | Manganese-containing pseudocatalase | K217 |
| TTHA0557 | Superoxide dismutase [Mn] | K6, K104, K105, K118, K120 |
| TTHA0706 | Cation-transporting ATPase | K70, K197 |
| TTHA1028 | Thiosulfate sulfurtransferase | K207, K216 |
| TTHA1477 | TrkA domain proteina | K92 |
| Secondary metabolite biosynthesis, transport, and catabolism | ||
| TTHA0223 | Type 11 methyltransferasea | K176 |
| TTHA0483 | Probable transcriptional regulator, ArsR family | K4 |
| TTHA0809 | Probable 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase | K154 |
| General function prediction only | ||
| TTHA0474 | Acetoin utilization protein AcuB (acetoin dehydrogenase) | K62 |
| TTHA0829 | Putative acetoin utilization protein, acetoin dehydrogenase | K50 |
| TTHA0972 | Phenylacetic acid degradation protein PaaA | K230 |
| Translation | ||
| TTHA0142 | Peptide chain release factor 2 | K335 |
| TTHA0161 | Leucyl-tRNA synthetase | K20, K205, K215, K468, K653 |
| TTHA0162 | 30S ribosomal protein S1 | K105 |
| TTHA0210 | 50S ribosomal protein L12 | K65, K87, K90, K95, K105, K112 |
| TTHA0246 | 50S ribosomal protein L1 | K85 |
| TTHA0250 | 50S ribosomal protein L33 | K17, K38 |
| TTHA0251 | Translation elongation factor EF-Tu.B | K3 |
| TTHA0366 | Aspartyl/glutamyl-tRNA amidotransferase subunit B | K256 |
| TTHA0543 | Glycyl-tRNA synthetase | K322, K443, K475 |
| TTHA0551 | Translation initiation factor 3 (IF-3) | K65 |
| TTHA0712 | Histidyl-tRNA synthetase | K10 |
| TTHA0858 | Ribosome recycling factor (Rrf) | K4, K112, K145 |
| TTHA0860 | Elongation factor Ts (EF-Ts) | K22, K120 |
| TTHA0875 | Seryl-tRNA synthetase | K66 |
| TTHA1067 | Isoleucyl-tRNA synthetase (IleRS) | K878 |
| TTHA1125 | Elongation factor P (EF-P) | K42 |
| TTHA1139 | Polynucleotide phosphorylase | K532 |
| TTHA1169 | Valyl-tRNA synthetase (ValRS) | K97, K845 |
| TTHA1294 | Ribosomal subunit interface protein | K122, K165 |
| TTHA1298 | Methionyl-tRNA synthetase | K429 |
| TTHA1397 | 30S ribosomal protein S20 | K48, K54 |
| TTHA1399 | Tyrosyl-tRNA synthetase (TyrRS) | K328 |
| TTHA1485 | Peptide chain release factor 1a | K4, K107 |
| TTHA1663 | 50S ribosomal protein L17 | K78 |
| TTHA1667 | 30S ribosomal protein S13 | K46, K79 |
| TTHA1675 | 30S ribosomal protein S5 | K145 |
| TTHA1678 | 30S ribosomal protein S8 | K46 |
| TTHA1680 | 50S ribosomal protein L5 | K47 |
| TTHA1684 | 30S ribosomal protein L29 | K8 |
| TTHA1695 | Elongation factor G (EF-G) | K10, K381, K513 |
| TTHA1696 | 30S ribosomal protein S7 | K53 |
| TTHA1697 | 30S ribosomal protein S12 | K111 |
| TTHA1875 | Threonyl-tRNA synthetase | K62, K249 |
| Post-translational modification, protein turnover, chaperones | ||
| Protein refolding | ||
| TTHA0271 | 60 kDa chaperonin, GroEL | K41, K42, K50, K116, K121, K129, K131, K169, K325, K334, K336, K337, K349, K362, K388, K429, K430, K440, K529 |
| TTHA0272 | 10 kDa chaperonin, GroES | K26, K28, K45, K66 |
| TTHA0614 | Trigger factor | K67 |
| TTHA1480 | Small heat shock protein, HSP20 family | K15, K123 |
| TTHA1484 | Small heat shock protein, HSP20 family | K8 |
| TTHA1487 | ATP-dependent Clp protease, ClpB | K424, K433, K448, K499, K510, K558, K630 |
| TTHA1491 | Chaperone protein DnaK (heat shock protein 70) | K84, K126, K245, K514 |
| Stress response | ||
| TTHA0370 | Thioredoxin reductase related protein | K108 |
| TTHA0593 | Probable thiol-disulfide isomerase/thioredoxin | K140 |
| TTHA1300 | Putative bacterioferritin comigratory protein/thiol peroxidase | K114 |
| TTHA1482 | Bacterioferritin | K69 |
| TTHA1625 | Osmotically inducible protein OsmC | K90, K120 |
| TTHA1635 | Iron-sulfur cluster biosynthesis protein IscA | K115 |
| TTHA1714 | Heme peroxidasea | K149 |
| TTHA1838 | SufC protein (ATP-binding protein) | K124, K149, K243 |
| TTHA1840 | SufD protein (membrane protein) | K14, K25, K28, K41, K42, K109, K336, K415 |
| TTHB162 | Crispr-associated ramp cmr1 familya | K359 |
| TTHB190 | Crispr-associated protein cse4a | K303, K318, K360 |
| Transcription | ||
| TTHA0008 | Phage shock protein A | K74 |
| TTHA0101 | Transcriptional repressor, TetR family | K185 |
| TTHA0248 | Transcription antitermination protein NusG | K27 |
| TTHA0701 | N utilization substance protein A (NusA) | K143, K182 |
| TTHA1664 | DNA-directed RNA polymerase α chain | K7, K270 |
| TTHA1812 | DNA-directed RNA polymerase β′ chain (RpoC) | K428 |
| TTHA1813 | DNA-directed RNA polymerase β chain (RpoB) | K86 |
| Replication, recombination, and repair | ||
| TTHA0180 | DNA polymerase III α subunit | K505 |
| TTHA1349 | DNA-binding protein HU | K23, K28, K68, K72, K76 |
| TTHA1583 | Site-specific DNA-methyltransferase | K338 |
| Intracellular trafficking and secretion | ||
| TTHA0365 | Type IV pilus assembly protein, PilT | K347 |
| TTHA0443 | Putative membrane protein | K298 |
| TTHA1251 | Preprotein translocase SecA subunit | K649 |
| Cell cycle control, mitosis, and meiosis | ||
| TTHA0564 | Cell division initiation protein DivIVA | K112 |
| TTHA1816 | Rod shape-determining protein MreB | K224 |
| Cell wall/membrane biogenesis | ||
| TTHA0850 | SpoVS related protein | K26 |
| General function prediction only | ||
| TTHA0051 | Conserved hypothetical protein | K8 |
| TTHA0420 | Putative flavoprotein | K84 |
| TTHA0810 | Metallo-β-lactamase related protein | K239, K291 |
| TTHA0961 | Flavin reductase component (HpaC)a | K6 |
| TTHA1483 | Conserved putative protein | K45, K147, K221 |
| TTHA1662 | Conserved hypothetical protein | K85 |
| Function unknown | ||
| TTHA1048 | Conserved hypothetical protein | K303 |
| TTHA1554 | Conserved hypothetical protein | K26 |
| TTHB059 | Hypothetical protein | K42 |
a The definition of protein function was updated using Gene Ontology annotation and KEGG Orthology.