Fig. 7.

Ubiquitination of cellular components during mitotic exit. A, enrichment analysis of the D-box and the KEN box in mitotic-exit-specific hits compared with non-phase-specific hits and a dataset of known APC/C substrates curated from the literature (57). The sequence of each protein was scanned for the presence of potential D-box (RXXL) and KEN motifs. The significance of enrichments was plotted as −log10(p values) calculated using a binomial test (null hypothesis: the frequency of observing D-box and KEN degrons is not greater in our dataset than in the whole human proteome) and indicated the presence of new APC/C substrates in our dataset, as even after removing known APC/C substrates from our dataset of mitotic-exit-specific hits we observed enrichment of degrons relative to the nonspecific dataset. B, interactions among proteins specifically ubiquitinated during mitotic exit. Protein interaction data were obtained using PSICQUIC (64) and plotted using Cytoscape (71).