Figure 1.

14,15-EET elicits transient receptor potential vanilloid type 1 (TRPV1)-dependent Ca²⁺ influx in human microvascular endothelial cells (HMECs). (A) HMECs were treated with 14,15-EET (10 nM) for the indicated times. Fluorescence images were photographed by fluorescence microscopy at 10 min after 14,15 EET treatment. (B) HMECs were pre-treated with Ca²⁺ free medium, EGTA (500 μM), an extracellular Ca²⁺ chelator, or BAPTA/AM (10 μM), an intracellular Ca²⁺ chelator, for 2 h then with 14,15-EET (10 nM) for 10 min. (C) HMECs were pre-treated with the TRPV1 pharmacological antagonist capsazepine (CPZ, 10 μM) or SB366791 (10 μM), for 2 h and then incubated with 14,15-EET (10 nM) for 10 min. The intracellular level of Ca²⁺ was analyzed by Fluo-8 calcium assay. Data are mean ± SEM from 5 independent experiments. ^*, P<0.05 versus vehicle; ^#, P<0.05 versus 14,15-EET.