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. 2014 Sep 6;10(9):990–996. doi: 10.7150/ijbs.9832

Figure 2.

Figure 2

The essential role of TRPV1 in 14,15-EET-induced Ca2+ influx in human embryonic kidney 293 (HEK293) cells. (A) HEK293 cells were transfected with vector or TRPV1 WT plasmid, then treated with 14,15-EET (10 nM) or capsaicin (10 μM) for 10 min. (B) After transfection with TRPV1 WT plasmid, HEK293 cells were pretreated with the TRPV1 antagonist CPZ (10 μM) or SB366791 (10 μM) for 2 h, then 14,15-EET (10 nM) for 10 min. (C) HEK293 cells were transfected with TRPV1 WT, TRPV1-Y671K or TRPV1-Y671D plasmid and then treated with 14,15-EET (10 nM) for 10 min. The intracellular level of Ca2+ was measured by Fluo-8 calcium assay. Fluorescence images were photographed by fluorescence microscopy. Data are mean ± SEM. *, P<0.05 versus vector or TRPV1 WT transfection alone; #, P<0.05 versus TRPV1 WT + 14,15-EET.