Figure 3.

TRPV1 is required for 14,15-EET-induced nitric oxide (NO) production. (A) HMECs were treated with 0, 2.5, 5, 10 nM 14,15-EET for 24 h or (B) treated with 10 nM 14,15-EET for indicated times. HMECs were pre-treated with Ca²⁺ free medium (C), 10 μM CPZ (D), or 10 μM SB366791 (E) for 2 h, then incubated in 10 nM 14,15-EET for 24 h. (F and G) HMECs were pretreated with 14,15-EE5ZE for 2 h and then incubated with 10 nM 14,15-EET for 10 min or 24 h. The intracellular level of Ca²⁺ was measured by Fluo-8 calcium assay and the level of nitrite in culture medium was measured by Griess's reagent. Data are mean ± SEM from 5 independent experiments. ^*, P<0.05 versus 0 min or vehicle; ^#, P<0.05 versus 14,15-EET.