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. 2014 Sep 6;10(9):1007–1017. doi: 10.7150/ijbs.9997

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© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.

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The p63+ BCs in the developed mouse prostate contain epithelial stem-like cells. A. Experimental flowchart. Three mice were used for each experiment (n=3). G1-G4 indicate sphere generations 1-4. The percentages of blue spheres (BSs), which are the β-gal+ spheres derived from the marked p63+ BCs, are indicated. B. X-gal-stained prostate tissues prepared from vehicle or tamoxifen-treated p63-CreERT2×Rosa26R mice. C. X-gal-stained epithelial spheres formed from isolated prostate epithelial cells of tamoxifen-treated p63-CreERT2×Rosa26R mice. White (WS) and blue (BS) spheres are indicated. D. A tamoxifen-treated epithelial sphere developed from the isolated prostate epithelial cells of untreated p63-CreERT2×Rosa26R mice. E. A blue second-generation sphere developed from re-culturing cells of the tamoxifen-treated first-generation spheres.