Figure 1.

Tissue swelling during oxygen and glucose deprivation (OGD) exposure. Brain slices were perfused with control aCSF until OGD exposure was initiated at time t=0 min. A. Representative images of brain slices from R+A+ and SARA animals. For each pair of images the top panel is the transmitted light image prior to OGD exposure. The hippocampal CA1 region and cerebral cortex are labeled for anatomical reference. The lower panel is a pseudocolor image of the same slice showing percent changes in light transmission after 30 min of OGD calculated on a pixel-by-pixel basis. A color calibration scale for the magnitude of the change in light transmission is shown in the figure insets. B. Mean IOS values from R+A+ and SARA animals before and during OGD exposure measured from regions of interest in cerebral cortex and hippocampal CA1 region. IOS is defined as the intensity of transmitted light during OGD treatment expressed as a percent of the value measured prior to the start of OGD. Values are the mean ± SEM for slices from 12 animals C. IOS values measured in cerebral cortex and the hippocampal CA1 region after 30 min of IOS exposure for brain slices from R+A+ and SARA animals with and without various drug treatments. The mean IOS from regions of interest in the cerebral cortex and hippocampal CA1 region for the final 5 images during OGD exposure was calculated for each slice and these results averaged to give the mean ± SEM values shown. The number of animals for each group is in the parentheses. Log-transformed data were analyzed by ANOVA with post hoc Dunnett’s test. For slices from SARA animals given no drug treatment, * indicates values that were significantly different from that measured in slices from R+A+ animals with no drug treatment. † indicates values significantly different from those measured in slices from animals of the same genotype with no drug treatment.