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. Author manuscript; available in PMC: 2015 Jul 25.

Published in final edited form as: Neuroscience. 2014 May 9;273:39–51. doi: 10.1016/j.neuroscience.2014.04.060

Nox2 and Nox4 expression following oxygen and glucose deprivation (OGD) exposure. Slices were perfused with control aCSF or OGD conditions for 30 min and then prepared for western blot analysis. Expression levels of Nox2 (A) and Nox4 (B) in brain slices from wild type (WT), R+A+, and SARA animals following control or OGD exposure are shown relative to expression levels of β-actin. C shows Nox expression in brain slices following OGD as a percent of the expression measured contemporaneously in brain slices from the same animal exposed to control conditions. Values are the mean ± SEM of data from 5 R+A+ animals, 5 wild type animals, and 4 SARA animals. Representative bands from western blots for Nox2, Nox4, and β-actin are shown under each bar in A and B. Data were analyzed by ANOVA with post hoc Neuman-Keuls test for multiple comparisons. * indicates values which are significantly different from those measured slices from wild type animals given the same perfusion treatment. † indicates values which are significantly different from those measured in slices from animals of the same genotype exposed to control conditions. For slices from SARA animals, ‡ indicates values which are significantly different from those measured in slices from R+A+ animals exposed to the same perfusion treatment.

Nox2 and Nox4 expression following oxygen and glucose deprivation (OGD) exposure. Slices were perfused with control aCSF or OGD conditions for 30 min and then prepared for western blot analysis. Expression levels of Nox2 (A) and Nox4 (B) in brain slices from wild type (WT), R+A+, and SARA animals following control or OGD exposure are shown relative to expression levels of β-actin. C shows Nox expression in brain slices following OGD as a percent of the expression measured contemporaneously in brain slices from the same animal exposed to control conditions. Values are the mean ± SEM of data from 5 R+A+ animals, 5 wild type animals, and 4 SARA animals. Representative bands from western blots for Nox2, Nox4, and β-actin are shown under each bar in A and B. Data were analyzed by ANOVA with post hoc Neuman-Keuls test for multiple comparisons. * indicates values which are significantly different from those measured slices from wild type animals given the same perfusion treatment. † indicates values which are significantly different from those measured in slices from animals of the same genotype exposed to control conditions. For slices from SARA animals, ‡ indicates values which are significantly different from those measured in slices from R+A+ animals exposed to the same perfusion treatment.