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. Author manuscript; available in PMC: 2015 Jul 9.
Published in final edited form as: Cell Host Microbe. 2014 Jul 9;16(1):31–42. doi: 10.1016/j.chom.2014.06.008

Figure 4. DC-SIGN-Raf-1 signaling inhibits dephosphorylation of RIG-I and Mda5.

Figure 4

(A–D) RIG-I phosphorylation at Ser8 or Thr170 and Mda5 phosphorylation at Ser88 in DCs left unstimulated; or 3 h after stimulation by crosslinking with isotype or DC-SIGN-specific antibodies (A,B), in the absence or presence of Raf inhibitor GW5074 (B); or 8 or 16 h after rMVKS infection or 3 h after or rMVIC323EGFP(1) in the absence or presence of Raf-1 inhibition via GW5074 (C) or Raf-1 silencing (D), as determined by flow cytometry.

Data are representative of at least four (A,B,C (rMVIC323, 8 h rMVKS), two (C (16 h rMVKS)) or three (D) independent experiments. See also Figure S2.